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DiO perchlorate [3,3’-Dioctadecyloxacarbocyanine perchlorate]

商品编号： 22066-10 mg

1198元/10mg

DiO perchlorate [3,3’-Dioctadecyloxacarbocyanine perchlorate]详细说明书

详细说明书 DiO perchlorate [3,3’-Dioctadecyloxacarbocyanine perchlorate]MSDS

DiO perchlorate [3,3’-Dioctadecyloxacarbocyanine perchlorate]MSDS

MSDS

DiO perchlorate [3,3’-Dioctadecyloxacarbocyanine perchlorate]技术资料

技术资料

产品介绍产品参数附件下载

产品介绍

DiO perchlorate [3,3-Dioctadecyloxacarbocyanine perchlorate]

Features and Biological Applications

DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He–Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.

目录号	产品名称	规格	分子量	Ex/Em	推荐滤光器*
DiD
22031	DiD iodide	25mg	959.91	644/663 nm	XF47-Omega, 31023-Chroma
22033	DiD labeling solution	10 mL	959.91	644/663 nm	XF47-Omega, 31023-Chroma
DiO
22046	DiOC6(3) iodide	100 mg	572.52	482/504 nm	XF23-Omega, 31001-Chroma
22066	DiO perchlorate	100 mg	881.7	484/501nm	XF23-Omega, 31001-Chroma
DiI
22101	DiI iodide	100 mg	961.32	549/565 nm	XF32-Omega, 31002-Chroma
22102	DiI perchlorate	100 mg	933.87	549/565 nm	XF32-Omega, 31002-Chroma
22103	DiI triflate	100 mg	983.48	549/565 nm	XF32-Omega, 31002-Chroma
DiR**
22070	DiR iodide	10 mg	1013.39	748/780 nm	XF112-Omega, 41009-Chroma
DiS
22073	DiSC2(3)	25 mg	492.44	560/571 nm	XF32-Omega, 31002-Chroma
22076	DiSC3(5)	100 mg	546.53	660/675 nm	XF47-Omega, 31023-Chroma

**DiR的发射波长肉眼不可见，所以需要配备CCD镜头或其他的近红外检测仪器。

1. Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ. (2006) Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials. J Biomed Mater Res B Appl Biomater.

2. Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K. (2006) In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope. Invest Ophthalmol Vis Sci, 47, 2943.

3. Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A. (2006) Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing. J Biomed Opt, 11, 050507.

4. Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C. (2006) Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius. Hypertension, 48, 482.

5. Wertz A, Rossler W, Obermayer M, Bickmeyer U. (2006) Functional neuroanatomy of the rhinophore of Aplysia punctata. Front Zool, 3, 6.

6. Harrison TA, Perry KM, Hoover DB. (2005) Regional cardiac ganglia projections in the guinea pig heart studied by postmortem DiI tracing. Anat Rec A Discov Mol Cell Evol Biol, 285, 758.

7. Huesa G, Anadon R, Yanez J. (2003) Afferent and efferent connections of the cerebellum of the chondrostean Acipenser baeri: a carbocyanine dye (DiI) tracing study. J Comp Neurol, 460, 327.

8. Kiyohara Y, Endo K, Ide C, Mizoguchi A. (2003) A novel morphological technique to investigate a single climbing fibre synaptogenesis with a Purkinje cell in the developing mouse cerebellum: DiI injection into the inferior cerebellar peduncle. J Electron Microsc (Tokyo), 52, 327.

9. Levai O, Strotmann J. (2003) Projection pattern of nerve fibers from the septal organ: DiI-tracing studies with transgenic OMP mice. Histochem Cell Biol, 120, 483.

特别提醒：

1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。

2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！

产品参数

Name	DiO perchlorate [3,3’-Dioctadecyloxacarbocyanine perchlorate]
CAT#	22066-10mg	CAS#	34215-57-1
Storage#	−20°C Sealed & desiccated & Minimized light exposure	Shelf Life#	24 months
Ex(nm)#	484	Em(nm)#	501
MW#	881.70	Solvent#	DMF or DMSO

Name	DiO perchlorate [3,3’-Dioctadecyloxacarbocyanine perchlorate]
CAT#	22066-10mg
CAS#	34215-57-1
Storage#	−20°C Sealed & desiccated & Minimized light exposure
Shelf Life#	24 months
Ex(nm)#	484
Em(nm)#	501
MW#	881.70
Solvent#	DMF or DMSO

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