Non-toxic nucleic acid gel stains
SafeRed® Nucleic Acid Gel Stain is the third generation nonmutagenic fluorescent nucleic acid gel stain to replace the highly toxic traditional ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. The third part’s Ames test by WuXi Pharmacy shows that SafeRed and DuGreen are nonmutagenic for gel staining. DuGreen and SafeRed Nucleic Acid Gel Stain are highly sensitive than EtBr either as precast gel stains or post gel stains. We can directly replace EB with SafeRed® without changing the existing imaging system since SafeRed® and EB have the similar spectra. SafeRed® can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. Gel staining with SafeRed® is compatible with downstream applications such as gel extraction and cloning. SafeRed® is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.