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Label it® FAM SE
Succinimidyl esters are proven to be the best reagents for amine modifications because the amide bonds that are formed are essentially identical to, and as stable as the natural peptide bonds. These reagents are generally stable and show good reactivity and selectivity with aliphatic amines. There are few factors that need be considered when SE compounds are used for conjugation reaction.
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产品编号
116(6-FAM SE)-113(5-FAM SE)
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抗体荧光标记试剂及标记服务
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产品描述
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Label it® FAM, SE [Carboxyfluorescein, succinimidyl ester, FITC-NHS]
Label it® is the registered trademark and strictly forbidden to be used without authorization.
Fluorescent Dye Carboxylic Acids and Their Succinimidyl Esters
Succinimidyl esters are proven to be the best reagents for amine modifications because the amide bonds that are formed are essentially identical to, and as stable as the natural peptide bonds. These reagents are generally stable and show good reactivity and selectivity with aliphatic amines. There are few factors that need be considered when SE compounds are used for conjugation reaction:
1). Solvents:For the most part, reactive dyes are hydrophobic molecules and should be dissolved in anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO).
2). Reaction pH:The labeling reactions of amines with succinimidyl esters are strongly pH dependent. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the terminal amines of proteins and the e-amino groups of lysines. Thus amine acylation reactions are usually carried out above pH 7.5. Protein modifications by succinimidyl esters can typically be done at pH 7.5-8.5, whereas isothiocyanates may require a pH 9.0-10.0 for optimal conjugations.
3).Reaction Buffers:Buffers that contain free amines such as Tris and glycine and thiol compounds must be avoided when using an amine-reactive reagent. Ammonium salts (such as ammonium sulfate and ammonium acetate) that are widely used for protein precipitation must also be removed (such as viadinlysis) before performing dye conjugations.
4). Reaction Temperature:Most conjugations are done at room temperature. However, either elevated or reduced temperature may be required for a particular labeling reaction.
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] CAS# 92557-81-8
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Features and Biological Applications6-FAM, SE is the amine-reactive succinimidyl ester of single isomer 6-FAM acid. It is one of the most popular green fluorescent reagents used for labeling nucleotides and nucleic acids. Compared to 5-FAM, 6-FAM is less often used to prepare small molecules. |
5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] CAS# 92557-80-7
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Features and Biological Applications5-FAM, SE is the amine-reactive succinimidyl ester of single isomer 5-FAM acid. It is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules.
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5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] CAS# 117548-22-8
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Features and Biological Applications5(6)-FAM, SE is the amine-reactive succinimidyl ester of FAM acid. It is favored over FITC in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. FITC-labeled nucleotides and peptides tend to deteriorate more quickly than the corresponding FAM conjugates. We found that FAM reagents can be used to substitute FITC reagents in most biological applications. |
References
1. Evans NA, et al. (2001). Visualizing differences in ligand-induced beta-arrestin-GFP interactions and trafficking between three recently characterized G protein-coupled receptors. J Neurochem77, 476-85.
2. Nasarabadi S, et al.(1999). Simultaneous detection of TaqMan probes containing FAM and TAMRA reporter fluorophores. Biotechniques27, 1116-8.
特别提醒：
1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。
2) 本公司所有产品必须由合格专业技术人员操作同时佩戴口罩/手套/实验服并遵守生物实验室安全操作规程！
产品参数
| Name | Label it® FAM, SE [Carboxyfluorescein, succinimidyl ester, FITC-NHS] | ||
|---|---|---|---|
| CAT# | 116(6-FAM SE)-113(5-FAM SE)-110(56-FAM SE) | CAS# | |
| Storage# | -20°C Sealed & desiccated & Minimized light exposure | Shelf Life# | 24 months |
| Ex(nm)# | 495 | Em(nm)# | 517 |
| MW# | 473.39 | Solvent# | DMF or DMSO |
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