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6-CR6G; 5-CR6G; 5,6-CR6G

332(6-CR6);331( 5-CR6G) ; 330-1195元/25mg
CR6G is the purified single isomer of 5(6)-CR6G. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates. This isomer is predominantly used to label small molecules, peptides and proteins.
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332(6-CR6);331( 5-CR6G) ; 330
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6-CR6G [6-Carboxyrhodamine 6G, hydrochloride]

Cat#

Size

Price

MW

Abs

Em

Soluble in

Storage 

1003

5mg

¥1050

494.97

520 nm

547 nm

DMSO

4ºC

 

Features and Biological Applications
6-CR6G is the other purified single isomer of 5(6)-CR6G. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates.  Complimentary to 5-CR6G, 6-CR6G is predominantly used for labeling nucleotides and nucleic acids.

 

 

 

5-CR6G [5-Carboxyrhodamine 6G, hydrochloride]

Cat#

Size

Price

MW

Abs

Em

Soluble in

Storage 

1002

10 mg

¥1050

494.97

518 nm

544 nm

DMSO

4ºC

 

Features and Biological Applications
5-CR6G is the purified single isomer of 5(6)-CR6G. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates. This isomer is predominantly used to label small molecules, peptides and proteins.
 

 

 

 

5(6)-CR6G [5-(and-6)-Carboxyrhodamine 6G, hydrochloride]

Cat#

Size

Price

MW

Abs

Em

Soluble in

Storage 

1001

25 mg

¥1195

494.97

519 nm

544 nm

DMSO

4ºC

 

Features and Biological Applications
5(6)-CR6G is the mixture of two carboxy rhodamine 6G isomers. It is used to modify amino and hydroxy groups using EDC-mediated couplings when there are difficulties in using 5(6)-CR6G, SE. The excitation and emission wavelengths of rhodamine 6G fall between those of fluorescein and tetramethylrhodamine derivatives, making 5(6)-CR6G another color choice for the multicolor fluorescence imaging applications.
 

 

 

References
1.  Seo, T.S., et al., Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides. Proc Natl Acad Sci USA, 2005, 102, 5926-31.
2.  Hung, S.C., et al., Energy transfer primers with 5- or 6-carboxyrhodamine-6G as acceptor chromophores. Anal Biochem,1996, 238, 165-70.
3. Chiu, D.T., et al., Injection of ultrasmall samples and single molecules into tapered capillaries. Anal Chem, 997. 69, 1801-7.
4. Hung SC, et al. (1997). Optimization of spectroscopic and electrophoretic properties of energy transfer primers. Anal Biochem1997. 252, 78-88.
5. Hung SC, et al. (1996). Energy transfer primers with 5- or 6-carboxyrhodamine-6G as acceptor chromophores. Anal Biochem238, 165-70.
6. Seo, T.S., et al., Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides.Proc Natl Acad Sci U S A2005, 102, 5926-31.
7.  Dietrich, A., et al., Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: A comparative study of ensemble and single-molecule data.J Biotechnol2002, 82, 211-31.
8. Arezi, B., et al., Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant.J Mol Biol2002, 322, 719-29.
9. Hung SC, et al. (1997). Optimization of spectroscopic and electrophoretic properties of energy transfer primers. Anal Biochem252, 78-88.
 

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产品参数

Name 5(6)-CR6G [5-(and-6)-Carboxyrhodamine 6G, hydrochloride]
CAT# 330-25mg CAS#  
Storage# -20°C Sealed & desiccated & Minimized light exposure Shelf Life# 24 months
Ex(nm)# 519 Em(nm)# 544
MW# 494.97  Solvent# DMSO
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