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5'-Tetrachlorofluorescein phosphoramidite
5’-fluorescein-CE phosphoramidite, derived from the single isomer 6-carboxyfluorescein, 5’-hexachlorofluorescein-CE phosphoramidite (HEX) and 5’-tetrachlorofluorescein-CE phosphoramidite (TET) can all be used to efficiently label an oligonucleotide at the 5’-end. Labeling the 3’-end of an oligo with fluorescein can be achieved using 3’-Fluorescein CPGs. Standard cleavage and deprotection with ammonium hydroxide liberates the fluorescein-labeled oligo when using any of these supports.
6-TAMRA CPG *1000 Å*
The light-absorbing properties of TAMRA, and spectral overlap with the used fluorophores - including FAM, HEX, TET and JOE.
TAMRA is a FRET acceptor for the dual labeled FRET probes such as Molecular Beacons. TAMRA has been used extensively for real-time PCR and other molecular diagnostic applications.
Oligonucleotides can be labeled with TAMRA using two distinct methodologies.
Oligonucleotides synthesized using UltraMILD monomers can also be labeled directly with TAMRA, either internally by substituting any suitable dT residue with TAMRA-dT-CE phosphoramidite, or at the 3'-end using 3'-TAMRA CPG support.
TAMRA is a FRET acceptor for the dual labeled FRET probes such as Molecular Beacons. TAMRA has been used extensively for real-time PCR and other molecular diagnostic applications.
Oligonucleotides can be labeled with TAMRA using two distinct methodologies.
Oligonucleotides synthesized using UltraMILD monomers can also be labeled directly with TAMRA, either internally by substituting any suitable dT residue with TAMRA-dT-CE phosphoramidite, or at the 3'-end using 3'-TAMRA CPG support.
6-JOE, SE
6-JOE has similar absorption and emission spectra to those of rhodamine 6G, and its SE is predominantly used in automated DNA sequencing. It is also used to label nucleotides.
6-TET, SE
6-TET, SE is a popular amino-reactive fluorescent probe that is widely used in nucleic acid sequencing and related research.
6-HEX, SE
6-HEX, SE is the amine-reactive succinimidyl ester of 6-HEX acid. It is widely used in nucleic acid sequencing and related research.
Chemical Phosphorylation Reagent I (CPR I)
This reagent can be used to create the 3'-phosphate by adding it as the first addition to the support in Labeling Oligonucleotides with Fluorescent Dyes. After ammonia deprotection the oligo will have the 3'-phosphate attached to the 2nd base added during synthesis. Both the support base and the CPR are cleaved. The DMT-group is removed during the ammonium hydroxide deprotection and thus is not available for poly-pak purification.
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