The light-absorbing properties of TAMRA, and spectral overlap with the used fluorophores - including FAM, HEX, TET and JOE.
TAMRA is a FRET acceptor for the dual labeled FRET probes such as Molecular Beacons. TAMRA has been used extensively for real-time PCR and other molecular diagnostic applications.
Oligonucleotides can be labeled with TAMRA using two distinct methodologies.
Oligonucleotides synthesized using UltraMILD monomers can also be labeled directly with TAMRA, either internally by substituting any suitable dT residue with TAMRA-dT-CE phosphoramidite, or at the 3'-end using 3'-TAMRA CPG support.