Annexin V-FITC/PI Dead Cell Apoptosis Detection Kit
The Annexin V-FITC Apoptosis assay kit detects apoptotic cells by flow cytometry. The annexins are a group of homologous proteins which bind phospholipids in the presence of calcium. Annexin V-FITC is a fluorescent probe which binds to phosphatidylserine in the presence of calcium. Apoptosis, or programmed cell death, is a mechanism of cells used to negatively select cells that are deleterious to the host. The cellular changes involved in the process include loss of phospholipid asymmetry during the early stages of apoptosis. In living cells, phosphatidylserine is transported to the inside of the lipid bilayer by the Mg-ATP dependent enzyme, aminophospholipid translocase. At the onset of apoptosis, phosphatidyl- serine, which is normally found on the internal part of the plasma membrane, becomes translocated to the external portion of the membrane. The phosphatidyl- serine becomes available to bind to the annexin V-FITC conjugate in the presence of calcium. The procedure consists of
Product ID
2020
SPEC
400元/20T;800/50T;1200元/100T