Super Green I for PCR

   Super Green I is the ideal substitute for Sybr Green, Super Green is a very sensitive dye for the detection of double stranded DNA (dsDNA), So it  has been widely used in non-specific detection of amplification in realtime qPCR experiments. The double-strand DNA-specific SYBR Green I fluorescent reporter offers distinct advantages. SYBR Green I dye is inexpensive, easy to use, and sensitive. Well-designed primers must be used in SYBR Green quantitative RT-PCR reactions because SYBR Green I dye will detect nonspecific products, resulting in an overestimation of the target concentration.

1. The flowing table 1 is our SYBR Green I RT-PCR Reagents Kit recipe for reference only. Please optimize it by yourself. 

2. On ice, prepare a 2x master mix containing no DNA, by mixing the components in the following order: water, DMSO, Taq polymerase buffer, dNTPs, MgCl2, Sybr Green, Taq polymerase.

3. Transfer 2x QuantiTect SYBR Green PCR Master Mix to tubes or plates. Add RNase-free water. Mix the individual solutions.

4. Prepare a reaction mix according to Table 2.

   Due to the hot start, it is not necessary to keep samples on ice during reaction setup or while programming the real-time cycler.

          PCR Reaction Setup:  Table 2

Program your real-time cycler according to the program outlined in Table 3

NOTE: Always wear lab coats, gloves and goggles when working with our products although they are low-risk chemicals for R&D only.