Hoechst 33342

中文名：二苯甲亚胺, 三氯化氢2'-(4-乙基苯基)-5-(4-甲基-1-哌嗪基)-2,5'-二-1H-苯并咪唑

英文名：2’-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bi-1H-benzimidazole, trihydrochloride

别名：Hoechst 33342，bisBenzimide H 33342，HOE 33342 

结构式：

性质：1. 外观：黄绿色粉末    2. 纯度：≥99%（HPLC）超纯级； 分子式：C27H28N6O ·3HCl；分子量：561.93；CAS#：23491-52-3

Description:

Chemical Name: Bisbenzimide, 2-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl) -2,5-bi-1H-benzimidazole trihydrochloride

Appearance: Yellow green powder                                   
MW: C₂₇H₃₁Cl₃N₆O=561.93

Storage Condition : 0-5^oC, protect from light
Shipping Condition : Ambient temperature

Product Description

   Hoechst dyes are cell membrane permeable and stain DNA to emit intense blue fluorescence. They bind to DNA in the minor groove of poly-AT sequence rich areas. Both Hoechst 33342 and Hoechst 33258 are water-soluble and stable in aqueous solutions. The excitation and emission wavelengths of Hoechst-DNA complex are 350 nm and 460 nm, respectively.

Staining Procedure

1. First prepare the stock HOECHST 33342 Dye solution: 1.9 mM Hoechst 33342 aqueous solution (1mg HOECHST 33342 Dye Powder in 1 ml dH₂O)

2. Prepare 10-50 μM Hoechst dye solution with PBS or an appropriate buffer to dilute the stock solution.

3. Add Hoechst dye solution with 1/10 of the volume of cell culture medium to the cell culture.

4. Incubate the cell at 37 °C for 10-20 min.                                              

5. Wash cells twice with PBS or an appropriate buffer.

6. Observe the cells under a fluorescence microscope with 350 nm excitation and 460 nm emission filters.

a) Since Hoechst dyes may be carcinogenic, extreme care is necessary during handling.
b) Or you may replace the culture medium with 1/10 concentration of Hoechst dye buffer solution. 

Safety warnings and precautions 

 NOTE: The qualified persons should always wear lab coats, gloves and goggles when working with our products although they are low-risk chemicals for R&D only.