DuGreen^®Nucleic Acid Gel Stain 

SafeRed^® and DuGreen^® are registered trademark and strictly forbidden to be used without authorization

Description

DuGreen^®(the ideal substitute for Sybr Green and 10000x GelGreen) is a kind of new generation of fluorescent nucleic acid gel stain designed to replace the highly toxic ethidium bromide (EtBr). Our Ames test confirms that DuGreen® are nonmutagenic at concentrations well above their working concentrations used for gel staining. DuGreen^® is highly sensitive than EtBr either as precast gel stains or post gel stains.

DuGreen^®has UV absorption between 250nm and 300nm and a strong absorption peak centered around 500nm and is compatible with either a 254nmUV transilluminator or a gel reader equipped with visible light excitation(such as a 488nm laser-based gel scanner or a Dark Reader).

DuGreen^® 10,000X in DMSO is a concentrated DuGreen® solution that can be diluted 10,000 times for use in precast gel staining for ~3,300 times for use in post gel staining according to the procedures described below. One vial(0.5ml) of 10,000X solution can be used to prepare at 100 precast minigels or post-stain at least 100 minigels.

Gel staining with DuGreen^® is compatible with downstream applications such as gel extraction and cloning. DuGreen® is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.

Features

1) Relative Safety: Nonmutagenic and noncytotoxic

2) Easy disposal: Safe to dispose in the drain

3) Good Compatibility: Spectrally compatible with all the existing instruments

4) High Sensitivity: Higher signal but lower background

6) High Stability: can be stored at RT and microwavable

Post-staining Protocol

1)   Run gels as usual according to your standard protocol.

2)   Dilute the DuGreen^®10,000X stock reagent~3,300 fold to make a 3X staining solution in H₂O. Note: including 0.1M NaCl in the staining solution enhances sensitivity, but may promote dye precipitation if the staining solution is reused.

3)   Carefully place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 3X staining solution to submerge the gel.

4)   Agitate the gel gently at room temperature for~30 minutes.

5)   Image the stained gel with a 254nm transilluminator, a Dark Reader or a similar transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR filter or FITC filter.

6)   Staining solution can be reused at least 2~3times. Store staining solution at room temperature protected from light.

Pre-cast Protocol

1)       Prepare molten agarose gel solution using your standard protocol.

2)       Dilute the DuGreen^®10,000X stock reagent into the molten agarose gel solution at 1:10,000 and mix thoroughly. DuGreen^®can be added while the gel solution is still hot.

3)       Cast the gel and allow it to solidify. Any leftover gel solution may be stored and reheated later for additional gel casting.DuGreen^®precast gels may be stored at 4°C for later use.

4)       Load samples and run the gels using your standard protocol.

5)       Image the stained gel with a 254nm transilluminator, a Dark Reader or a similar transilluminator or a laser-based gel scanner using a long path green filter such as a SYBR filter or FITC filter.

Note: The pre-cast protocol is not recommended for polyacrylamide gels.

NOTE: Always wear lab coats, gloves and goggles when working with our products although they are low-risk chemicals for R&D only.