Super Gold DNA Gel Stain

Description

Super Gold is the ideal substitute for Sybr Gold in DNA gel stain with specifically designed for reduced mutagenicity, making it safer to replace the highly toxic ethidium bromide (EtBr) for staining DNA in agarose or acrylamide gels. Super Gold DNA gel stain showed no or very low mutagenic activity at concentrations well above working concentrations used for gel staining. Super Gold Nucleic Acid Gel Stain is highly sensitive than EtBr either as precast gel stains or post gel stains.

   DNA bands stained with Super Gold DNA gel stain  can be detected using a standard UV transilluminator, a visible-light transilluminator, or a laser-based scanner. The stain is also suitable for staining RNA in gels

Super Gold Nucleic Acid Gel Stain 10,000X in DMSO is a concentrated Super Gold solution that can be diluted 10,000 times for use in precast gel staining for ~3,300 times for use in post gel staining according to the procedures described below. One vial(0.5ml) of 10,000X solution can be used to prepare at 100 precast minigels or post-stain at least 100 minigels.

Gel staining with Super Gold is compatible with downstream applications such as gel extraction and cloning. Super Gold is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.

Bound to nucleic acids, Super Gold DNA Gel  stain has fluorescence excitation maxima at 280 and 502 nm, and an emission maximum at 530 nm

Storage

Store the Super Gold DNA gel stain at any temperature between 2 to 25°C. It must be completely thawed and mixed if the Super Gold is frozen at low temperatures before using. Repeated freeze-thawing has minimal impact on product performance. Super Gold DNA gel stain showed no or very low mutagenic activity.

NOTE: Always wear lab coats, gloves and goggles when working with our products although they are low-risk chemicals for R&D only.

Features

1) Relative Safety: Nonmutagenic and noncytotoxic 2020-49(6)-938

2) Easy disposal: Safe to dispose in the drain

3) Good Compatibility: Spectrally compatible with all the existing instruments

4) High Sensitivity: Higher signal but lower background

6) High Stability: can be stored at RT and microwavable

Post-staining Protocol

1)   Run gels as usual according to your standard protocol.

2)   Dilute the Super Gold 10,000X stock reagent~3,300 fold to make a 3X staining solution TAE or TBE buffer prior to use.

3)   Carefully place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 3X staining solution to submerge the gel.

4)   Agitate the gel gently at room temperature for~30 minutes.

5)   Image the stained gel with a 254nm transilluminator, a Dark Reader or a similar transilluminator, or a laser-based gel scanner using a long path green filter such as a SUPERfilter or GelStar filter.

6)   Staining solution can be reused at least 2~3times. Store staining solution at room temperature protected from light.

7)   Stained gels can be viewed using a standard 300 nm transilluminator, a 254 nm epi- or transilluminator, or a blue-light transilluminator

Pre-cast Protocol

1)        Prepare molten agarose gel solution using your standard protocol.

2)        Dilute the Super Gold 10,000X stock reagent into the molten agarose gel solution at 1:10,000 and mix thoroughly. Super Gold can be added while the gel solution is still warm (50~60°C). For example if you run TBE gels and require 30 mL of molten agarose for your tray, add 3 µL of 10,000X Super Gold stain concentrate to 30 mL of 1X TBE.

3)        Cast the gel and allow it to solidify. Any leftover gel solution may be stored and reheated later for additional gel casting Super Gold precast gels may be stored at 4°C for later use.

4)        Load samples and run the gels using your standard protocol.

5)        Image the stained gel with a 254nm transilluminator, a Dark Reader or a similar transilluminator or a laser-based gel scanner using a long path green filter such as a SUPER filter or GelStar filter.

6)        Stained gels can be viewed using a standard 300 nm transilluminator, a 254 nm epi- or transilluminator, or a blue-light transilluminator.

Note: The pre-cast protocol is not recommended for polyacrylamide gels.